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Enzyme engineering services

VTU´s high-throughput enzyme engineering service for the improvement of the properties of your enzyme
Supporting you in product innovation, VTU offers fast and reliable engineering and screening of improved enzyme variants.

The key advantage of using VTU´s Pichia system for the generation of enzyme variants is that engineering is performed in the final production host. Therefore, potential bottlenecks in changing the host system from primary screen to final enzyme production are eliminated, thus accelerating the commercialization of your improved enzyme. In addition, VTU´s established high-throughput microscale cultivation and screening protocols facilitate the rapid identification of improved protein variants with specific properties. Application of VTU´s Pichia toolbox ensures production of high-value enzymes at lower manufacturing costs.

Next to classical objectives like increasing activity towards a specific substrate, any kind of increased stability upon incubation at e.g. varying temperatures, differing pH-values or diverse reaction matrices has been successfully achieved.

Libraries of gene variants are created by error-prone PCR and/or site directed mutagenesis potentially supported by molecular modeling. The formation of single copy integrations is favored by using appropriate amounts of linearized DNA paired with the ideal selection pressure in order to allow for highest comparability in screenings. Using VTU´s established 96-deep-well plate cultivation for homogeneous growth of individual transformants, high-throughput screening of thousands of gene variants in parallel is carried out using spectrophotometric or fluorometric activity analysis. In addition, a pre-screening on solid media to eventually cultivate only a selected set of transformants can be envisaged.

Key benefits

  • High-throughput-screening for engineered variants using a eukaryotic production system
  • Engineering for improved variants performed in final production host
  • VTU´s established high-throughput microscale cultivation and screening protocols facilitate the fast and reliable identification of protein variants with desired enhanced properties
  • Short timelines and high flexibility
  • Projects tailored to your needs

 

Deliverables

  • Samples of optimized protein variants
  • Production strains of engineered variants
  • Comprehensive report

 

Technology

  • Library generation using classical and tailored mutagenesis tools
  • Transformation of library into Pichia pastoris favoring single copy integration events
  • Cultivation using VTU´s established high-throughput microscale protocol
  • Screening based on liquid spectrophotometric or fluorometric assays
  • Pre-screening on solid media possible
Further Services

Pichia pastoris protein expression excellence

Be competitive with high-quality VTU Pichia proteins, high-productivity strains & robust and scalable bioprocesses
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Time-saving protein expression studies

Assess the potential - VTU´s streamlined feasibility studies

 

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Comprehensive strain development

Unlock the potential by applying VTU´s full protein production toolbox

 

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03

Bioprocess development

Straightforward fermentation development, optimization and characterization services
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04

Protein purification and DSP development

Protein purification and establishment of analytical procedures and product characterization
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Cell banking                    

Preparation and characterization of research cell banks
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Enzyme engineering

High-throughput engineering and screening of enzyme variants
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News

What About Pichia?  

Read our latest article "What about Pichia"  reviewing recent advances in ...
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Meet us at  

EVENTS 2017 - CPhI 2017, 24-26 October, Frankfurt, Germany, Booth 41G30  
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Downloads

Company folder and brochures

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