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Assessment of expression potential

Straightforward determination of expression potential & rapid production of protein samples
You instantly need a few mgs of recombinant protein?


Expression in other systems is troublesome or failed?


Expression in commercially available yeasts was not satisfying?


Are you searching for a powerful expression system system suitable for industrial protein production?



In a lean feasibility study we will elucidate the expression potential of your recombinant protein in minimum time. Starting from an optimized synthetic gene sequence, results will be available within 3 - 4 weeks after cloning, transformation and screening giving you an immediate answer for a reliable go/no-go decision. Positive candidates from micro-scale screening can be directly transferred to bioreactor cultivation enabling both the production of initial protein samples as well as a further assessment of the potential for improvement.

After positive assessment we provide yield optimization by applying VTU´s comprehensive production strain development.

Key benefits

  • Fast assessment of expression feasibility and potential for further improvement
  • Immediate answers for a reliable go/no-go decision
  • Results from micro-scale screening transferable to bioreactor cultivation
  • Rapid generation of first samples and protein production strains

 Deliverables

  • Small scale production of non-GMP protein samples
  • Glycerol stocks of expression strains
  • Comprehensive report

Technology

  • Methanol-induced and methanol-free processes
  • Codon optimization for maximum protein yields
  • Customized screening size applying a subset of VTU´s AOX1 promoter library
  • Helper factor co-expression
  • High-throughput cultivation and screening in 96-deep well plates
  • Cultivation of most productive clones in 1 l or 7.5 l bioreactors for rapid protein production

Further services

Pichia pastoris protein expression excellence

Be competitive with high-quality VTU Pichia proteins, high-productivity strains & robust and scalable bioprocesses
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Time-saving protein expression studies

Assess the potential - VTU´s streamlined feasibility studies

 

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02

Comprehensive strain development

Unlock the potential by applying VTU´s full protein production toolbox

 

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03

Bioprocess development

Straightforward fermentation development, optimization and characterization services
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04

Protein purification and DSP development

Protein purification and establishment of analytical procedures and product characterization
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05

Cell banking                    

Preparation and characterization of research cell banks
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06

Enzyme engineering

High-throughput engineering and screening of enzyme variants
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News

What About Pichia?  

Read our latest article "What about Pichia"  reviewing recent advances in ...
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Meet us at  

EVENTS 2017 - CPhI 2017, 24-26 October, Frankfurt, Germany, Booth 41G30  
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Downloads

Company folder and brochures

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