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Protein Sample Purification and DSP Development

High yield, scalable and cost efficient purification processes coupled with a broad toolbox of established analytical methods
Do you need a highly purified sample of your protein for your functional studies?

Are you seeking to establish protein purification in fairly short time?

Do you look for a robust scalable downstream process?

 

In order to provide you with a highly pure sample of your protein in short time, we will set up a purification process that will yield milligram to gram quantities of pure protein sample from a Pichia culture supernatant. Based on our experience and taking into account the properties of your protein, we will scout a set of promising methods to come up with a purification strategy focusing on speed and purity. Purification methods applied are chosen from standard chromatography techniques such as ion exchange or hydrophobic interaction as well as affinity chromatography in combination with ultrafiltration steps.

According to your requirements, we will also take downstream processing one step further providing full process development for a robust scalable process. In close coordination with our customers, we will evaluate capacities, maximize yield and purity, establish clearance of impurities and understand how subsequent steps are interlinked. Comprehensive documentation and support guarantee neat transfer of the optimized downstream process to your production site.

All DSP activities are evidently accompanied by analytical characterization of process intermediates and purified product. Established in house methods include 1- and 2D-PAGE, capillary electrophoresis, HPLC, Western Blot, ELISA as well as methods to quantify process related impurities, including HCP and host cell DNA.

 

Key benefits

  • Highly pure protein samples according to your specifications
  • Short time lines and high flexibility
  • Projects tailored to your needs – from rapid setup to fully developed processes


Deliverables

  • Purified protein samples (small scale non-GMP production)
  • Scalable purification process
  • Full documentation to support process transfer and regulatory filings


Technology

  • Highly versatile equipment to perform systematic small scale process development as well as small to medium scale production
  • Proven strategies for rapid process setup
  • Established analytical tools for protein product as well as process related impurities, including HCP and host cell DNA

 

Further Services

Pichia pastoris protein expression excellence

Be competitive with high-quality VTU Pichia proteins, high-productivity strains & robust and scalable bioprocesses
01

Time-saving protein expression studies

Assess the potential - VTU´s streamlined feasibility studies

 

Details
02

Comprehensive strain development

Unlock the potential by applying VTU´s full protein production toolbox

 

Details
03

Bioprocess development

Straightforward fermentation development, optimization and characterization services
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04

Protein purification and DSP development

Protein purification and establishment of analytical procedures and product characterization
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05

Cell banking                    

Preparation and characterization of research cell banks
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06

Enzyme engineering

High-throughput engineering and screening of enzyme variants
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News

What About Pichia?  

Read our latest article "What about Pichia"  reviewing recent advances in ...
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Meet us at  

EVENTS 2017 - CPhI 2017, 24-26 October, Frankfurt, Germany, Booth 41G30  
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Downloads

Company folder and brochures

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